Antibacterial effect of new phage for treatment of Pseudomonas aeruginosa Keratitis in an animal model
Seyed Hashem Daryabari1 *, Ruhollah Dorostkar2 , Seyyed Morteza Hosseini Imeni3
- Baghiatollah University of Medical Sciences, Tehran, Islamic Republic of Iran
- Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
- Shahid Beheshti University Of Medical Sciences
Abstract: Pseudomonas aeruginosa is one of the common bacterial agents in keratitis and is
resistant to drugs. Due to the difference in the mechanism of action of antibacterial
compounds, phage therapy can be a therapeutic candidate to deal with drug-resistant
infections.
Methods: The purpose of this study is to isolate, identify, and investigate the
antimicrobial ability of a phage sample to treat keratitis caused by P. aeruginosaThe isolated phage belongs to the Straboviridae family and the Caudoviricetes order.
Results: The anti-biofilm effect of phage was observed on clinical isolates. IC50 of phage (on
human corneal epithelial cell) were determined as 10-1. The synergistic effect of
ciprofloxacin and phage in the form of eye drops was observed in the improvement of
keratitis caused by P. aeruginosa.
Conclusion: The results obtained in this study indicated that phage can be effective in controlling
bacterial keratitis infection. However, combined antibiotic and phage treatment have a
synergistic effect
